Red Xylem and Higher Lignin Extractability by Down-Regulating a Cinnamyl Alcohol Dehydrogenase in Poplar.
Identifieur interne : 004A33 ( Main/Exploration ); précédent : 004A32; suivant : 004A34Red Xylem and Higher Lignin Extractability by Down-Regulating a Cinnamyl Alcohol Dehydrogenase in Poplar.
Auteurs : M. Baucher [Belgique] ; B. Chabbert ; G. Pilate ; J. Van Doorsselaere ; M. T. Tollier ; M. Petit-Conil ; D. Cornu ; B. Monties ; M. Van Montagu ; D. Inze ; L. Jouanin ; W. BoerjanSource :
- Plant physiology [ 1532-2548 ] ; 1996.
Abstract
Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in the biosynthesis of the lignin precursors, the monolignols. We have down-regulated CAD in transgenic poplar (Populus tremula X Populus alba) by both antisense and co-suppression strategies. Several antisense and sense CAD transgenic poplars had an approximately 70% reduced CAD activity that was associated with a red coloration of the xylem tissue. Neither the lignin amount nor the lignin monomeric composition (syringyl/guaiacyl) were significantly modified. However, phloroglucinol-HCl staining was different in the down-regulated CAD plants, suggesting changes in the number of aldehyde units in the lignin. Furthermore, the reactivity of the cell wall toward alkali treatment was altered: a lower amount of lignin was found in the insoluble, saponified residue and more lignin could be precipitated from the soluble alkali fraction. Moreover, large amounts of phenolic compounds, vanillin and especially syringaldehyde, were detected in the soluble alkali fraction of the CAD down-regulated poplars. Alkaline pulping experiments on 3-month-old trees showed a reduction of the kappa number without affecting the degree of cellulose degradation. These results indicate that reducing the CAD activity in trees might be a valuable strategy to optimize certain processes of the wood industry, especially those of the pulp and paper industry.
DOI: 10.1104/pp.112.4.1479
PubMed: 12226459
PubMed Central: PMC158080
Affiliations:
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Tollier</name></author><author><name sortKey="Petit Conil, M" sort="Petit Conil, M" uniqKey="Petit Conil M" first="M." last="Petit-Conil">M. Petit-Conil</name></author><author><name sortKey="Cornu, D" sort="Cornu, D" uniqKey="Cornu D" first="D." last="Cornu">D. Cornu</name></author><author><name sortKey="Monties, B" sort="Monties, B" uniqKey="Monties B" first="B." last="Monties">B. Monties</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M." last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D." last="Inze">D. Inze</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L." last="Jouanin">L. Jouanin</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W." last="Boerjan">W. 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Baucher</name><affiliation wicri:level="4"><nlm:affiliation>Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology (M.B., J.V.D., M.V.M., D.I., W.B.), and Laboratoire Associe de I'Institut National de la Recherche Agronomique (France), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Belgium (M.B., D.I.).</nlm:affiliation><orgName type="university">Université de Gand</orgName><country>Belgique</country><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Chabbert, B" sort="Chabbert, B" uniqKey="Chabbert B" first="B." last="Chabbert">B. Chabbert</name></author><author><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G." last="Pilate">G. Pilate</name></author><author><name sortKey="Van Doorsselaere, J" sort="Van Doorsselaere, J" uniqKey="Van Doorsselaere J" first="J." last="Van Doorsselaere">J. Van Doorsselaere</name></author><author><name sortKey="Tollier, M T" sort="Tollier, M T" uniqKey="Tollier M" first="M. T." last="Tollier">M. T. Tollier</name></author><author><name sortKey="Petit Conil, M" sort="Petit Conil, M" uniqKey="Petit Conil M" first="M." last="Petit-Conil">M. Petit-Conil</name></author><author><name sortKey="Cornu, D" sort="Cornu, D" uniqKey="Cornu D" first="D." last="Cornu">D. Cornu</name></author><author><name sortKey="Monties, B" sort="Monties, B" uniqKey="Monties B" first="B." last="Monties">B. Monties</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M." last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D." last="Inze">D. Inze</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L." last="Jouanin">L. Jouanin</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W." last="Boerjan">W. Boerjan</name></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in the biosynthesis of the lignin precursors, the monolignols. We have down-regulated CAD in transgenic poplar (Populus tremula X Populus alba) by both antisense and co-suppression strategies. Several antisense and sense CAD transgenic poplars had an approximately 70% reduced CAD activity that was associated with a red coloration of the xylem tissue. Neither the lignin amount nor the lignin monomeric composition (syringyl/guaiacyl) were significantly modified. However, phloroglucinol-HCl staining was different in the down-regulated CAD plants, suggesting changes in the number of aldehyde units in the lignin. Furthermore, the reactivity of the cell wall toward alkali treatment was altered: a lower amount of lignin was found in the insoluble, saponified residue and more lignin could be precipitated from the soluble alkali fraction. Moreover, large amounts of phenolic compounds, vanillin and especially syringaldehyde, were detected in the soluble alkali fraction of the CAD down-regulated poplars. Alkaline pulping experiments on 3-month-old trees showed a reduction of the kappa number without affecting the degree of cellulose degradation. These results indicate that reducing the CAD activity in trees might be a valuable strategy to optimize certain processes of the wood industry, especially those of the pulp and paper industry.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">12226459</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>112</Volume><Issue>4</Issue><PubDate><Year>1996</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Red Xylem and Higher Lignin Extractability by Down-Regulating a Cinnamyl Alcohol Dehydrogenase in Poplar.</ArticleTitle><Pagination><MedlinePgn>1479-1490</MedlinePgn></Pagination><Abstract><AbstractText>Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in the biosynthesis of the lignin precursors, the monolignols. We have down-regulated CAD in transgenic poplar (Populus tremula X Populus alba) by both antisense and co-suppression strategies. Several antisense and sense CAD transgenic poplars had an approximately 70% reduced CAD activity that was associated with a red coloration of the xylem tissue. Neither the lignin amount nor the lignin monomeric composition (syringyl/guaiacyl) were significantly modified. However, phloroglucinol-HCl staining was different in the down-regulated CAD plants, suggesting changes in the number of aldehyde units in the lignin. Furthermore, the reactivity of the cell wall toward alkali treatment was altered: a lower amount of lignin was found in the insoluble, saponified residue and more lignin could be precipitated from the soluble alkali fraction. Moreover, large amounts of phenolic compounds, vanillin and especially syringaldehyde, were detected in the soluble alkali fraction of the CAD down-regulated poplars. Alkaline pulping experiments on 3-month-old trees showed a reduction of the kappa number without affecting the degree of cellulose degradation. These results indicate that reducing the CAD activity in trees might be a valuable strategy to optimize certain processes of the wood industry, especially those of the pulp and paper industry.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Baucher</LastName><ForeName>M.</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology (M.B., J.V.D., M.V.M., D.I., W.B.), and Laboratoire Associe de I'Institut National de la Recherche Agronomique (France), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Belgium (M.B., D.I.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chabbert</LastName><ForeName>B.</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>G.</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Van Doorsselaere</LastName><ForeName>J.</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Tollier</LastName><ForeName>M. T.</ForeName><Initials>MT</Initials></Author><Author ValidYN="Y"><LastName>Petit-Conil</LastName><ForeName>M.</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Cornu</LastName><ForeName>D.</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Monties</LastName><ForeName>B.</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Van Montagu</LastName><ForeName>M.</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Inze</LastName><ForeName>D.</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Jouanin</LastName><ForeName>L.</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>W.</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>9</Month><Day>13</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12226459</ArticleId><ArticleId IdType="pii">112/4/1479</ArticleId><ArticleId IdType="pmc">PMC158080</ArticleId><ArticleId IdType="doi">10.1104/pp.112.4.1479</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>EMBO J. 1991 Jul;10(7):1723-32</Citation><ArticleIdList><ArticleId IdType="pubmed">2050109</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1964 Apr;105:103-13</Citation><ArticleIdList><ArticleId IdType="pubmed">14165483</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 1975 Nov 1;59(1):9-15</Citation><ArticleIdList><ArticleId IdType="pubmed">1250</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1990 Nov;87(22):9057-61</Citation><ArticleIdList><ArticleId IdType="pubmed">11607118</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1990;41:455-96</Citation><ArticleIdList><ArticleId IdType="pubmed">11543592</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1996 Jan;110(1):3-13</Citation><ArticleIdList><ArticleId IdType="pubmed">12226169</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7608-12</Citation><ArticleIdList><ArticleId IdType="pubmed">8052628</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1994 Jan;104(1):75-84</Citation><ArticleIdList><ArticleId IdType="pubmed">12232063</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1995 Apr;7(4):407-16</Citation><ArticleIdList><ArticleId IdType="pubmed">7773015</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Top Microbiol Immunol. 1995;197:57-75</Citation><ArticleIdList><ArticleId IdType="pubmed">7493497</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId></ArticleIdList></Reference><Reference><Citation>Stain Technol. 1987 Jul;62(4):279-80</Citation><ArticleIdList><ArticleId IdType="pubmed">3307031</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 Dec;94(4):1743-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16667911</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Mar 25;17(6):2362</Citation><ArticleIdList><ArticleId IdType="pubmed">2468132</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W." last="Boerjan">W. Boerjan</name><name sortKey="Chabbert, B" sort="Chabbert, B" uniqKey="Chabbert B" first="B." last="Chabbert">B. Chabbert</name><name sortKey="Cornu, D" sort="Cornu, D" uniqKey="Cornu D" first="D." last="Cornu">D. Cornu</name><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D." last="Inze">D. Inze</name><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L." last="Jouanin">L. Jouanin</name><name sortKey="Monties, B" sort="Monties, B" uniqKey="Monties B" first="B." last="Monties">B. Monties</name><name sortKey="Petit Conil, M" sort="Petit Conil, M" uniqKey="Petit Conil M" first="M." last="Petit-Conil">M. Petit-Conil</name><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G." last="Pilate">G. Pilate</name><name sortKey="Tollier, M T" sort="Tollier, M T" uniqKey="Tollier M" first="M. T." last="Tollier">M. T. Tollier</name><name sortKey="Van Doorsselaere, J" sort="Van Doorsselaere, J" uniqKey="Van Doorsselaere J" first="J." last="Van Doorsselaere">J. Van Doorsselaere</name><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M." last="Van Montagu">M. Van Montagu</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Baucher, M" sort="Baucher, M" uniqKey="Baucher M" first="M." last="Baucher">M. Baucher</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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